Band-by-Band Densitometric Analysis of Synchrotron X-ray Footprinting Autoradiograms
نویسنده
چکیده
A nucleotide-by-nucleotide density fitting procedure has been successfully developed and has been applied to an analysis of a domain of the Tetrahymena ribozyme and a TBP-DNA interaction. This procedure is described in a paper that has been submitted for publication [Pastor, N., Weinstein, H., Jamison, E. & Brenowitz, M. A Detailed Interpretation of OH Radical Footprints in a TBP-DNA Complex Reveals the Role of Dynamics in the Mechanism of Sequence-Specific Binding.] In this paper a detailed comparison of the •OH footprint of a TBP-DNA complex is compared to the solvent accessibility of the DNA calculated from co-crystal structures and structures derived from molecular dynamics simulations.
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تاریخ انتشار 2000